We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Catalytic Performance of a Recombinant Organophosphate-Hydrolyzing Phosphotriesterase from Brevundimonas diminuta in the Presence of Surfactants.
- Authors
Chi, Meng-Chun; Liao, Ting-Yu; Lin, Min-Guan; Lin, Long-Liu; Wang, Tzu-Fan; Zhang, Baiyu; Liu, Bo
- Abstract
Phosphotriestease (PTE), also known as parathion hydrolase, has the ability to hydrolyze the triester linkage of organophosphate (OP) pesticides and chemical warfare nerve agents, making it highly suitable for environment remediation. Here, we studied the effects of various surfactants and commercial detergents on the esterase activity of a recombinant PTE (His6-tagged BdPTE) from Brevundimonas diminuta. Enzymatic assays indicated that His6-tagged BdPTE was severely inactivated by SDS even at lower concentrations and, conversely, the other three surfactants (Triton X-100, Tween 20, and Tween 80) had a stimulatory effect on the activity, especially at a pre-incubating temperature of 40 °C. The enzyme exhibited a good compatibility with several commercial detergents, such as Dr. Formula® and Sugar Bubble®. The evolution results of pyrene fluorescence spectroscopy showed that the enzyme molecules participated in the formation of SDS micelles but did not alter the property of SDS micelles above the critical micelle concentration (CMC). Structural analyses revealed a significant change in the enzyme's secondary structure in the presence of SDS. Through the use of the intentionally fenthion-contaminated Chinese cabbage leaves as the model experiment, enzyme–Joy® washer solution could remove the pesticide from the contaminated sample more efficiently than detergent alone. Overall, our data promote a better understanding of the links between the esterase activity of His6-tagged BdPTE and surfactants, and they offer valuable information about its potential applications in liquid detergent formulations.
- Subjects
CHOLINESTERASE reactivators; CRITICAL micelle concentration; SURFACE active agents; CHEMICAL warfare agents; TRITON X-100; CHINESE cabbage
- Publication
Catalysts (2073-4344), 2021, Vol 11, Issue 5, p597
- ISSN
2073-4344
- Publication type
Article
- DOI
10.3390/catal11050597