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- Title
Systematic Characterization of the Group 2 House Dust Mite Allergen in Dermatophagoides microceras.
- Authors
Hu, Rei-Hsing; Wu, Chia-Ta; Wu, Ting-Shuan; Yu, Feng-Yih; Ko, Jiunn-Liang; Lue, Ko-Huang; Liu, Yu-Fan
- Abstract
Background: Allergic asthma, a chronic airway inflammatory disease, is a critical public health problem. Indoor house dust mites (HDMs) could cause allergic asthma. The prevalence of sensitization to Dermatophagoides microceras (Der m) was approximately 80% and is related to the immunoglobulin E crossing-reactivity of mites belonging to the same genus, Dermatophagoides pteronyssinus (Der p) and Dermatophagoides farina (Der f). However, studies on Der m are scant. Methods: We used integrated OMICs approaches to identify and characterize the group 2 mite allergen-like protein in Der m (Der m 2). We established a Der m 2 -induced allergic asthma mouse model and treated the mice with a fungal immunomodulatory protein (FIP- fve) isolated from Flammulina veluptipes to evaluate the allergenicity of Der m 2 and the immunomodulatory effects of FIP- fve. Results: By performing de novo draft genome assembly and comparative genome analysis, we identified the putative 144-amino acid Der m 2 in silico and further confirmed its existence through liquid chromatography–tandem mass spectrometry. Der m 2 is a lipopolysaccharides (LPS)-binding protein. Thus, we examined the LPS-binding activity of recombinant Der m 2 by performing molecular docking analysis, co-immunoprecipitation (Co-IP), and a pull-down assay. Der m 2 elicited the production of pro-inflammatory cytokines, interleukin (IL)-6, and IL-8 in BEAS-2B cells, a human bronchial epithelial cell line, and induced airway hyperresponsiveness in mice. Furthermore, in mice sensitized with Der m 2 , the administration of FIP- fve in either the earlier stage or the late stage, FIP- fve alleviated allergic asthma by moderating airway inflammation and remodeling. Conclusions: Der m 2 induced inflammatory responses in cell and mouse models. FIP- fve alleviated inflammation in Der m 2 -induced asthma in mice by exerting an immunomodulatory effect.
- Subjects
DERMATOPHAGOIDES pteronyssinus; HOUSE dust mites; DERMATOPHAGOIDES; LIQUID chromatography-mass spectrometry; IMMUNOGLOBULIN E; VAN der Waals forces; FUNGAL proteins; ALLERGENS
- Publication
Frontiers in Cellular & Infection Microbiology, 2022, Vol 11, p1
- ISSN
2235-2988
- Publication type
Article
- DOI
10.3389/fcimb.2021.793559