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- Title
Influence of picloram and thidiazuron on high frequency plant regeneration in elite cultivars of wheat with long-term retention of morphogenecity using meristematic shoot segments.
- Authors
Sharma, V. K.; Hänsch, R.; Mendel, R. R.; Schulze, J.
- Abstract
Meristematics shoot segments (MSSs) were used to develop an efficient and reproducible regeneration system via multiple shoot differentiation in two elite cultivars of winter wheat. Cylindrical basal shoot segments, 3-4 mm in length, were excised from seedlings derived from mature embryos that had been geminated directly on MS-based medium containing growth regulators. These segments were further cultured on the same medium formulations. Out of 24 variations analysed, the combination of 2 mg/l 4-amino-3,5,6-trichloropicolinic acid (picloram) + 3 mg/l thidiazuron (TDZ) was found to be the most effective in inducing clumps of multiple shoots/buds with little callusing. Sectors of these clumps were (1) further multiplied by biweekly subculturing on 2 mg/l picloram + 2.5 mg/l TDZ leading to highly differentiating green clusters of buds without loss of morphogenesis for more than 12 months so far, and without albinism, or (2) transferred to 0.1 mg/l picloram + 1 mg/l TDZ for shoot development and elongation. The elongated shoots rooted on basal medium with 0.5 mg/l indole-3-butyric acid (IBA) were transferred to soil and showed normal growth and fertility. Both cultivars formed multiple shoots ranging from 28 to 33 per responding MSS after 10-12 weeks.
- Subjects
HERBICIDES; PICLORAM; REGENERATION (Botany); PLANT injuries; WHEAT; WINTER wheat; PLANT morphogenesis
- Publication
Plant Breeding, 2005, Vol 124, Issue 3, p242
- ISSN
0179-9541
- Publication type
Article
- DOI
10.1111/j.1439-0523.2005.01095.x