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- Title
Leucine Supplementation Does Not Attenuate Skeletal Muscle Loss during Leg Immobilization in Healthy, Young Men.
- Authors
Backx, Evelien M.P.; Horstman, Astrid M.H.; Marzuca-Nassr, Gabriel N.; van Kranenburg, Janneau; Smeets, Joey S.; Fuchs, Cas J.; Janssen, Anniek A.W.; de Groot, Lisette C.P.G.M.; Snijders, Tim; Verdijk, Lex B.; van Loon, Luc J.C.
- Abstract
Background: Short successive periods of physical inactivity occur throughout life and contribute considerably to the age-related loss of skeletal muscle mass. The maintenance of muscle mass during brief periods of disuse is required to prevent functional decline and maintain metabolic health. Objective: To assess whether daily leucine supplementation during a short period of disuse can attenuate subsequent muscle loss in vivo in humans. Methods: Thirty healthy (22 ± 1 y) young males were exposed to a 7-day unilateral knee immobilization intervention by means of a full leg cast with (LEU, <italic>n</italic> = 15) or without (CON, <italic>n</italic> = 15) daily leucine supplementation (2.5 g leucine, three times daily). Prior to and directly after immobilization, quadriceps muscle cross-sectional area (computed tomography (CT) scan) and leg strength (one-repetition maximum (1-RM)) were assessed. Furthermore, muscle biopsies were taken in both groups before and after immobilization to assess changes in type I and type II muscle fiber CSA. Results: Quadriceps muscle cross-sectional area (CSA) declined in the CON and LEU groups (<italic>p</italic> < 0.01), with no differences between the two groups (from 7712 ± 324 to 7287 ± 305 mm2 and from 7643 ± 317 to 7164 ± 328 mm2; <italic>p</italic> = 0.61, respectively). Leg muscle strength decreased from 56 ± 4 to 53 ± 4 kg in the CON group and from 63 ± 3 to 55 ± 2 kg in the LEU group (main effect of time <italic>p</italic> < 0.01), with no differences between the groups (<italic>p</italic> = 0.052). Type I and II muscle fiber size did not change significantly over time, in both groups (<italic>p</italic> > 0.05). Conclusions: Free leucine supplementation with each of the three main meals (7.5 g/d) does not attenuate the decline of muscle mass and strength during a 7-day limb immobilization intervention.
- Subjects
KNEE physiology; LEG physiology; MUSCLE physiology; SARCOPENIA; BIOPSY; COMPUTED tomography; DIETARY supplements; LEUCINE; QUADRICEPS muscle; CROSS-sectional method; TREATMENT duration; IN vivo studies; DISEASE risk factors
- Publication
Nutrients, 2018, Vol 10, Issue 5, p635
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu10050635