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- Title
Arduino-based automation of a DNA extraction system.
- Authors
Kyung-Won Kim; Mi-So Lee; Mun-Ho Ryu; Jong-Won Kim; Kim, Kyung-Won; Lee, Mi-So; Ryu, Mun-Ho; Kim, Jong-Won
- Abstract
There have been many studies to detect infectious diseases with the molecular genetic method. This study presents an automation process for a DNA extraction system based on microfluidics and magnetic bead, which is part of a portable molecular genetic test system. This DNA extraction system consists of a cartridge with chambers, syringes, four linear stepper actuators, and a rotary stepper actuator. The actuators provide a sequence of steps in the DNA extraction process, such as transporting, mixing, and washing for the gene specimen, magnetic bead, and reagent solutions. The proposed automation system consists of a PC-based host application and an Arduino-based controller. The host application compiles a G code sequence file and interfaces with the controller to execute the compiled sequence. The controller executes stepper motor axis motion, time delay, and input-output manipulation. It drives the stepper motor with an open library, which provides a smooth linear acceleration profile. The controller also provides a homing sequence to establish the motor's reference position, and hard limit checking to prevent any over-travelling. The proposed system was implemented and its functionality was investigated, especially regarding positioning accuracy and velocity profile.
- Subjects
NUCLEIC acid isolation methods; AUTOMATION; ARDUINO (Microcontroller); TIME delay systems; ACTUATORS; MOLECULAR biology; AUTOMATION equipment; DNA analysis; PSYCHOLOGICAL tests; PRODUCT design; FAMILY relations; MICROFLUIDIC analytical techniques; EQUIPMENT &; supplies
- Publication
Technology & Health Care, 2016, Vol 24, pS105
- ISSN
0928-7329
- Publication type
journal article
- DOI
10.3233/THC-151048