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- Title
Facilitating circular permutation using Restriction Free (RF) cloning.
- Authors
Bandyopadhyay, Boudhayan; Peleg, Yoav
- Abstract
Circular permutation is a powerful tool to test the role of topology in protein folding and function. Previous methods for generating circular permutants were based on rearranging gene elements using restriction enzymes-based cloning. Here, we present a Restriction Free (RF) approach to achieve circular permutation which is faster and more cost-effective.
- Subjects
CYCLIC permutations; CLONING; NUCLEOTIDE sequencing; GENETIC engineering; MOLECULAR biology
- Publication
PEDS: Protein Engineering, Design & Selection, 2018, Vol 31, Issue 3, p65
- ISSN
1741-0126
- Publication type
Article
- DOI
10.1093/protein/gzx061