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- Title
Stress hormones collaborate to induce lymphocyte apoptosis after high level spinal cord injury.
- Authors
Lucin, Kurt M.; Sanders, Virginia M.; Popovich, Phillip G.
- Abstract
Post-traumatic immune suppression renders individuals with spinal cord injury (SCI) susceptible to infection. Normally, proper immune function is regulated by collaboration between the sympathetic nervous system (SNS) and hypothalamic–pituitary–adrenal (HPA) axis and involves the controlled release of glucocorticoids (GCs) and norepinephrine (NE). Recently, we showed that after high thoracic (T3) SCI, aberrant levels of GCs and NE accumulate in the blood and spleen, respectively. These changes are associated with splenic atrophy, splenic leucopenia, increased intrasplenic caspase 3 levels, and suppressed B lymphocyte function. As GCs boost SNS function, in part by increasing the expression and affinity of β2 adrenergic receptors (β2ARs) while simultaneously preventing β2AR down-regulation, we predicted that surges in stress hormones (i.e., GCs and NE) in the blood and spleen of mice with high-level SCI would act concurrently to adversely affect lymphocyte function and survival. Here, we show that post-SCI concentrations of GCs enhance the sensitivity of lymphocytes to β2AR stimulation causing an increase in intracellular Bcl-2 interacting mediator of cell death (Bim) and subsequent apoptosis. In vivo, the combined antagonism of GC receptors and β2ARs significantly diminished lymphocyte Bim levels and SCI-induced splenic lymphopenia. Together, these data suggest that pharmacological antagonists of the HPA/SNS axes should be considered as adjunct therapies for ameliorating post-traumatic immune suppression in quadriplegics and high paraplegics.
- Subjects
HYPOTHALAMIC-pituitary-adrenal axis; LYMPHOCYTES; APOPTOSIS; SPINAL cord; NERVOUS system; NORADRENALINE; MUSCULAR atrophy
- Publication
Journal of Neurochemistry, 2009, Vol 110, Issue 5, p1409
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/j.1471-4159.2009.06232.x