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- Title
Protective effect of diet supplemented with rice prolamin extract against DNCB-induced atopic dermatitis in BALB/c mice.
- Authors
Hyun-Joong Yoon; Mi-Sun Jang; Hyun-Woo Kim; Dong-Up Song; Kwang-Il Nam; Choon-Sang Bae; Seong-Jin Kim; Seung-Rock Lee; Chang-Sub Ku; Dong-Il Jang; Bong-Whan Ahn
- Abstract
Background: Rice prolamin has been reported to possess antioxidative, anti-inflammatory and immune-promoting properties. This study is aimed to examine the protective effects of dietary rice prolamin extract (RPE) against dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD)-like skin lesions in mice. Methods: BALB/c mice were fed diet supplemented with 0-0.1 % RPE for 6 weeks. For the last 2 weeks, 1 % or 0.2 % DNCB was applied repeatedly to the back skin of mice to induce AD-like lesions. Following AD induction, the severity of skin lesions was examined macroscopically and histologically. In addition, the serum levels of IgE, IgG1 and IgG2a were determined by ELISA, and the mRNA expression of IL-4 and IFN-y in the skin was determined by real-time PCR. Results: Dietary RPE suppressed the clinical symptoms of DNCB-induced dermatitis as well as its associated histopathological changes such as epidermal hyperplasia and infiltration of mast cells and eosinophils in the dermis. RPE treatment also suppressed the DNCB-induced increase in transepidermal water loss. Dietary RPE inhibited the DNCB-induced enhancement of serum IgE and IgG1 levels, whereas it increased the serum IgG2a level in DNCB-treated mice. In addition, dietary RPE upregulated the IFN-y mRNA expression and downregulated the IL-4 mRNA expression in the skin of DNCB-treated mice. Conclusions: The above results suggest that dietary RPE exerts a protective effect against DNCB-induced AD in mice via upregulation of Th1 immunity and that RPE may be useful for the treatment of AD.
- Subjects
ANIMAL experimentation; ATOPIC dermatitis; DIETARY supplements; ENZYME-linked immunosorbent assay; HISTOLOGICAL techniques; IMMUNOGLOBULINS; MICE; POLYMERASE chain reaction; RESEARCH funding; RICE; STAINS &; staining (Microscopy); STATISTICS; PLANT extracts; DATA analysis; CONTROL groups; REVERSE transcriptase polymerase chain reaction; DATA analysis software; DESCRIPTIVE statistics; ONE-way analysis of variance
- Publication
BMC Complementary & Alternative Medicine, 2015, Vol 15, p1
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/s12906-015-0892-0