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- Title
Mycobacterium bovis BCG killed by extended freeze-drying induces an immunoregulatory profile and protects against atherosclerosis.
- Authors
Ovchinnikova, O. A.; Berge, N.; Kang, C.; Urien, C.; Ketelhuth, D. F. J.; Pottier, J.; Drouet, L.; Hansson, G. K.; Marchal, G.; Bäck, M.; Schwartz‐Cornil, I.; Lagranderie, M.
- Abstract
Objectives Atherosclerosis is an inflammatory disease of the arterial wall that leads to myocardial infarction and stroke. Regulatory T cells ( Tregs) and IL-10 exert significant anti-atherogenic effects in experimental models of atherosclerosis by modulating vascular inflammation. We have previously shown that Mycobacterium bovis BCG killed by extended freeze-drying ( EFD BCG) decreases lung and colon inflammation by recruiting IL-10-producing Tregs. Therefore, the aim of this study was to investigate the effect of EFD BCG on the development of atherosclerosis. Design We used two strains of atherosclerosis-prone mice: Ldlr −/− (four or six EFD BCG injections) and Apoe −/− (six injections). Results In both models, EFD BCG significantly reduced the size of atherosclerotic lesions, increased IL-10 production and reduced the serum levels of pro-inflammatory cytokines ( IL-6, IL-13, KC and tumour necrosis factor-α). Shortly after treatment with EFD BCG, the number of plasmacytoid dendritic cells ( pDCs) and Foxp3+ Tregs in the draining lymph nodes increased. EFD BCG also led to accumulation of Tregs, but not of pDCs in the spleen, and reduced activity of NF-κB and increased activity of PPAR-γ in both the spleen and vascular tissue of treated mice. Conclusion EFD BCG has atheroprotective effects through IL-10 production and Treg expansion. These findings support a novel approach to the prevention and treatment of atherosclerosis.
- Subjects
ATHEROSCLEROSIS treatment; MYCOBACTERIUM bovis; FREEZE-drying; IMMUNOREGULATION; INFLAMMATION; ATHEROSCLEROSIS prevention; MYOCARDIAL infarction risk factors; STROKE risk factors
- Publication
Journal of Internal Medicine, 2014, Vol 275, Issue 1, p49
- ISSN
0954-6820
- Publication type
Article
- DOI
10.1111/joim.12127