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- Title
GC-MS/MS Determination of Steroid Hormones in Urine Using Solid-Phase Derivatization as an Alternative to Conventional Methods.
- Authors
Temerdashev, Azamat; Nesterenko, Pavel; Dmitrieva, Ekaterina; Zhurkina, Kseniya; Feng, Yu-Qi
- Abstract
Solid-phase analytical derivatization (SPAD) is a promising hybrid sample preparation technique combining the clean-up and preconcentration of the sample in a single step. In this work, a novel SPAD method based on the preparation of trimethylsilyl (TMS) derivatives of steroid hormones (testosterone, estrone, DHT, estriol, estradiol, and progesterone) in Phenomenex Strata C18-E (100 mg, 1 mL) cartridges has been developed and applied for their GC-MS/MS determination in human urine samples. The proposed procedure allows the detection and quantification of steroids with limits of 1.0–2.5 and 2.5–5 ng/mL, respectively. These characteristics are comparable with those obtained with a conventional liquid–liquid extraction, while the recovery of analytes in the proposed SPAD procedure is higher. The major advantages of SPAD are a short derivatization time, high efficiency, and the possibility to automatize the procedure. However, its cost-effectiveness in routine practice is still questionable.
- Subjects
STEROID hormones; DERIVATIZATION; LIQUID-liquid extraction; URINE; ESTRIOL
- Publication
Molecules, 2022, Vol 27, Issue 18, p5796
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules27185796