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- Title
Optimization of Sample Preparation for Metabolomics Exploration of Urine, Feces, Blood and Saliva in Humans Using Combined NMR and UHPLC-HRMS Platforms.
- Authors
Martias, Cécile; Baroukh, Nadine; Mavel, Sylvie; Blasco, Hélène; Lefèvre, Antoine; Roch, Léa; Montigny, Frédéric; Gatien, Julie; Schibler, Laurent; Dufour-Rainfray, Diane; Nadal-Desbarats, Lydie; Emond, Patrick
- Abstract
Currently, most clinical studies in metabolomics only consider a single type of sample such as urine, plasma, or feces and use a single analytical platform, either NMR or MS. Although some studies have already investigated metabolomics data from multiple fluids, the information is limited to a unique analytical platform. On the other hand, clinical studies investigating the human metabolome that combine multi-analytical platforms have focused on a single biofluid. Combining data from multiple sample types for one patient using a multimodal analytical approach (NMR and MS) should extend the metabolome coverage. Pre-analytical and analytical phases are time consuming. These steps need to be improved in order to move into clinical studies that deal with a large number of patient samples. Our study describes a standard operating procedure for biological specimens (urine, blood, saliva, and feces) using multiple platforms (1H-NMR, RP-UHPLC-MS, and HILIC-UHPLC-MS). Each sample type follows a unique sample preparation procedure for analysis on a multi-platform basis. Our method was evaluated for its robustness and was able to generate a representative metabolic map.
- Subjects
BIOLOGICAL specimens; SALIVA; FECES; URINE; METABOLOMICS; STANDARD operating procedure
- Publication
Molecules, 2021, Vol 26, Issue 14, p4111
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules26144111