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- Title
Subacute Oral Administration of Clinacanthus nutans Ethanolic Leaf Extract Induced Liver and Kidney Toxicities in ICR Mice.
- Authors
Aliyu, Abdullahi; Shaari, Mohd Rosly; Ahmad Sayuti, Nurul Syahirah; Reduan, Mohd Farhan Hanif; Sithambaram, Shanmugavelu; Noordin, Mustapha Mohamed; Shaari, Khozirah; Hamzah, Hazilawati
- Abstract
This study investigated the leaves of Clinacanthus nutans for its bioactive compounds and acute and subacute toxicity effects of C. nutans ethanolic leaf extract (CELE) on blood, liver and kidneys of ICR mice. A total of 10 8-week-old female mice were divided into groups A (control) and B (2000 mg/kg) for the acute toxicity study. A single dose of 2000 mg/kg was administered to group B through oral gavage and mice were monitored for 14 days. In the subacute toxicity study, mice were divided into five groups: A (control), B (125 mg/kg), C (250 mg/kg), D (500 mg/kg) and E (1000 mg/kg). The extract was administered daily for 28 days via oral gavage. The mice were sacrificed, and samples were collected for analyses. Myricetin, orientin, isoorientin, vitexin, isovitexin, isookanin, apigenin and ferulic acid were identified in the extract. Twenty-eight days of continuous oral administration revealed significant increases (p < 0.05) in creatinine, ALT and moderate hepatic and renal necrosis in groups D and E. The study concluded that the lethal dose (LD50) of CELE in mice is greater than 2000 mg/kg and that repeated oral administrations of CELE for 28 days induced hepatic and renal toxicities at 1000 mg/kg in female ICR mice.
- Subjects
NEPHROTOXICOLOGY; HEPATOTOXICOLOGY; PROXIMAL kidney tubules; MICE; FERULIC acid; EXTRACTS
- Publication
Molecules, 2020, Vol 25, Issue 11, p2631
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules25112631