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- Title
A vaccine-matching assessment of different genetic variants of serotype O foot-and-mouth disease virus isolated in Ethiopia between 2011 and 2014.
- Authors
Tesfaye, Yeneneh; Khan, Fazlurrahman; Yami, Martha; Wadsworth, Jemma; Knowles, Nick J.; King, Donald P.; Gelaye, Esayas
- Abstract
The aim of this study was to assess the vaccine-matching and antigenic properties of foot-and-mouth disease virus (FMDV) isolates collected from Ethiopia between 2011 and 2014. Samples (n = 51) were collected from cattle and pigs with clinical signs consistent with foot-and-mouth disease (FMD) on farms in Debre-Berhan, Debre-Zeit/Bishoftu, Sidamo, Mekelle, and Addis Ababa. Infectious FMDV was isolated using BHK-21 cell cultures from 38 of the 51 field samples (74.5%). All of these FMDV-positive samples were characterized as serotype O, belonging to two East Africa topotypes (EA-3 and EA-4), and their VP1-encoding sequences demonstrated amino acid sequence variability encompassing 27 positions in comparison to the vaccine strain (O/ETH/38/2005) currently provided by the National Veterinary Institute of Ethiopia. One-dimensional virus neutralization test (1 dm VNT) results showed that O/ETH/38/2005 was antigenically matched to 10 of the 16 serotype O viruses. These findings indicate that the O/ETH/38/2005 vaccine strain can provide protection against outbreaks caused by the O/EA-3 topotype, although poorer vaccine-matching results for the O/EA-4 topotype reinforce the importance of using a good-quality vaccine with high coverage in the susceptible herds with supporting post-vaccination serosurveillance to ensure that sufficient antibody titers are generated in the vaccinated animals.
- Subjects
ETHIOPIA; VIRUS diseases; FOOT &; mouth disease; AMINO acid sequence; ANIMAL health surveillance; NEUTRALIZATION tests; SYMPTOMS; CELL culture
- Publication
Archives of Virology, 2020, Vol 165, Issue 8, p1749
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-020-04662-y