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- Title
Inhibitory effect of mizoribine on matrix metalloproteinase-1 production in synovial fibroblasts and THP-1 macrophages.
- Authors
Zhong, Binbin; Tajima, Michiko; Takahara, Hidenari; Nochi, Hiromi; Tamoto, Koichi; Tamura, Naoto; Kobayashi, Shigeto; Tamura, Yoshifumi; Ikeda, Makoto; Akimoto, Tomohiro; Yoshino, Shinichi; Hashimoto, Hiroshi
- Abstract
To investigate the mechanism of antirheumatic action of mizoribine (MZR), we examined the expression of matrix metalloproteinase-1 (MMP-1) and MMP-3 utilizing THP-1 derived macrophage-like cells (THP-1 macrophages) and human synovial fibroblasts (SFs). The cells were respectively stimulated with lipopolysaccharide (LPS) and interleukin-1β in the presence or absence of MZR in vitro. The concentrations of MMP-1 and MMP-3 in the supernatant were measured by enzyme-linked immunosorbent assay. The secretion of MMP-1 from SFs, as well as THP-1 macrophages, was inhibited by MZR in a dose-dependent manner. Furthermore, a quantitative real-time polymerase chain reaction revealed that MZR decreased the expression of MMP-1 messenger RNA. These findings may be an explanation for the clinical effect of MZR in patients with rheumatoid arthritis.
- Subjects
METALLOPROTEINASES; METALLOENZYMES; RHEUMATOID arthritis; ANTIRHEUMATIC agents; ANALGESICS
- Publication
Modern Rheumatology, 2005, Vol 15, Issue 4, p264
- ISSN
1439-7595
- Publication type
Article
- DOI
10.3109/s10165-005-0406-x