We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
EFFECT OF POLYMORPHISM G(129)R IN GROWTH DIFFERENTIATION FACTOR 9 GENE (GDF9) ON AWASSI EWES THAT BREED OUT OF SEASON.
- Authors
Abid, Ali Aziz; Younis, Laith Sofian; Rasheed, Saad Tawfiq
- Abstract
The present study was performed to test the linkage between GDF9 mutation (G(129)R) and seasonality in Awassi sheep. Thirty mature non-pregnant ewes were used in this study in the period between September/ 2018 to January/2019. Fifteen ewes were lambed at September and November/2018 and this was considered as the Seasonal group, and the Non- Seasonal group consisted of 15 ewes which were lambed in mid-December and January/2019. A double primers were utilized to magnificate the 1st exon of GDF9 gene by a polymerase chain reaction (PCR) after the DNA extraction was done from blood specimens. The single nucleutide polymorphism (SNP) were detected through sequencing ( by matching with the GDF9 gene sheep sequencing in NCBI data bases. The results revealed one single nucleotide polymorphism G(129)R in chromosome 5 of GDF9 gene (exon I) when compared with Sequence ID: FJ429111.1, without resulting in an amino acid. Two genotypes (GG and GA) were showed, and high significant differences (P< 0.001) were find between the genotypic frequencies of G(129)R locus. The results showed that the mutant heterozygote genotype (GA) recorded a higher significant rise (P< 0.001) in non-seasonal ewes (60%) as compared with the wild homozygote (GG) genotype (40%). In contrast, the genotype frequency (GG) recorded a higher significantly increment (P< 0.001) in seasonal ewes (63%) in comparison with (GA) genotype (37%). As a conclusion, exon I GDF9 gene polymorphism has an effect on breeding out of season in Awassi breed and the mutant genotype find mostly in out of season Awsassi ewes.
- Subjects
SINGLE nucleotide polymorphisms; AWASSI sheep; GENETIC mutation; DNA primers; EXONS (Genetics)
- Publication
Biochemical & Cellular Archives, 2019, Vol 19, Issue 2, p4649
- ISSN
0972-5075
- Publication type
Article
- DOI
10.35124/bca.2019.19.2.4649