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- Title
Seed hemicelluloses tailor mucilage properties and salt tolerance.
- Authors
Yang, Bo; Hofmann, Florian; Usadel, Björn; Voiniciuc, Cătălin
- Abstract
Summary: While Arabidopsis seed coat epidermal cells have become an excellent genetic system to study the biosynthesis and structural roles of various cell wall polymers, the physiological function of the secreted mucilaginous polysaccharides remains ambiguous. Seed mucilage is shaped by two distinct classes of highly substituted hemicelluloses along with cellulose and structural proteins, but their interplay has not been explored.We deciphered the functions of four distinct classes of cell wall polymers by generating a series of double mutants with defects in heteromannan, xylan, cellulose, or the arabinogalactan protein SALT‐OVERLY SENSITIVE 5 (SOS5), and evaluating their impact on mucilage architecture and seed germination during salt stress.We discovered that muci10 seeds, lacking heteromannan branches, had elevated tolerance to salt stress, while heteromannan elongation mutants exhibited reduced germination in calcium chloride (CaCl2). By contrast, xylan made by MUCILAGE‐RELATED21 (MUCI21) was found to be required for the adherence of mucilage pectin to microfibrils made by CELLULOSE SYNTHASE5 (CESA5) as well as to a SOS5‐mediated network.Our results indicate that the substitution of xylan and glucomannan in seeds can fine‐tune mucilage adherence and salt tolerance, respectively. The study of germinating seeds can thus provide insights into the synthesis, modification and function of complex glycans.
- Subjects
MUCILAGE; HEMICELLULOSE; GALACTOMANNANS; CYTOSKELETAL proteins; CALCIUM chloride; GLUCOMANNAN; GLYCANS
- Publication
New Phytologist, 2021, Vol 229, Issue 4, p1946
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.17056