We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Investigation of morin-induced insulin secretion in cultured pancreatic cells.
- Authors
Lin, Mang Hung; Hsu, Chia‐Chen; Lin, Jenshinn; Cheng, Juei Tang; Wu, Ming Chang
- Abstract
Morin is a flavonoid contained in guava that is known to reduce hyperglycemia in diabetes. Insulin secretion has been demonstrated to increase following the administration of morin. The present study is designed to investigate the potential mechanism(s) of morin-induced insulin secretion in the MIN6 cell line. First, we identified that morin induced a dose-dependent increase in insulin secretion and intracellular calcium content in MIN6 cells. Morin potentiated glucose-stimulated insulin secretion ( GSIS). Additionally, we used si RNA for the ablation of imidazoline receptor protein ( NISCH) expression in MIN6 cells. Interestingly, the effects of increased insulin secretion by morin and canavanine were markedly reduced in Si- NISCH cells. Moreover, we used KU14R to block imidazoline I3 receptor (I-3R) that is known to enhance insulin release from the pancreatic β-cells. Without influence on the basal insulin secretion, KU14R dose-dependently inhibited the increased insulin secretion induced by morin or efaroxan in MIN6 cells. Additionally, effects of increased insulin secretion by morin or efaroxan were reduced by diazoxide at the dose sufficient to open KATP channels and attenuated by nifedipine at the dose used to inhibit L-type calcium channels. Otherwise, phospholipase C ( PLC) is introduced to couple with imidazoline receptor (I-R). The PLC inhibitor dose-dependently inhibited the effects of morin in MIN6 cells. Similar blockade was also observed in protein kinase C ( PKC) inhibitor-treated cells. Taken together, we found that morin increases insulin secretion via the activation of I-R in pancreatic cells. Therefore, morin would be useful to develop in the research and treatment of diabetic disorders.
- Subjects
INSULIN; MORIN; INTRACELLULAR calcium; CANAVANINE; IMIDAZOLINES
- Publication
Clinical & Experimental Pharmacology & Physiology, 2017, Vol 44, Issue 12, p1254
- ISSN
0305-1870
- Publication type
Article
- DOI
10.1111/1440-1681.12815