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- Title
Galectin-12 is Involved in Corn Silk-Induced Anti-Adipogenesis and Anti-Obesity Effects.
- Authors
Hsu, Yu-An; Kuo, Yi-Hsin; Chen, Chih-Sheng; Chen, Ying-Chi; Huang, Chi-Chun; Chang, Ching-Yao; Lin, Chao-Jen; Lin, Cheng-Wen; Lin, Hui-Ju; Liu, Fu-Tong; Wan, Lei
- Abstract
Obesity is a significant risk factor for various diseases. It is a clinical condition caused by the excessive accumulation of fat, which has a negative impact on human health. Galactin-12 is an adipocyte-expressed protein and possesses adipocyte-inducing activity. We investigated the expression level of candidate proteins involved in galactin-12-mediated adipocyte differentiation pathway. We performed a high-throughput screening assay to monitor galectin-12 promoter activity using 105 traditional Chinese herbs. Corn silk extract and β -sitosterol reduced the expression of galactin-12 promoter in 3T3-L1 cells. In addition, corn silk extract and β -sitosterol decreased the level of lipid droplets and downregulated the gene and protein expression level of C/EBP β , C/EBP α , PPAR γ , Ap2, and adipsin in 3T3-L1 pre-adipocytes via AKT and ERK1/2 inhibition. In vivo study with the oral administration of corn silk extract and β -sitosterol in a mouse model showed a significant weight reduction and decrease in adipocytes in several organs such as the liver and adipose tissue. Taken together, corn silk extract and β -sitosterol may effectively reduce pre-adipocyte differentiation by inhibiting galectin-12 activity and exerting anti-obesity effects. These findings highlight the potential use of corn silk extract and β -sitosterol as potential candidates for the prevention and treatment of obesity.
- Subjects
ANTIOBESITY agents; ANALYSIS of variance; ANIMAL experimentation; BIOMARKERS; BIOCHEMISTRY; BLOOD sugar; CELL culture; CELL differentiation; CELL lines; CHOLESTEROL; CORN; CREATININE; FAT cells; GENE expression; GENETIC techniques; HERBAL medicine; HISTOLOGICAL techniques; KETOGENIC diet; LIPIDS; PHENOMENOLOGY; CHINESE medicine; MICE; ORAL drug administration; OXIDOREDUCTASES; POLYMERASE chain reaction; PROTEINS; RESEARCH funding; RNA; STATISTICS; TRIGLYCERIDES; WEIGHT loss; WESTERN immunoblotting; LEPTIN; HIGH throughput screening (Drug development); DATA analysis; ALBUMINS; DATA analysis software; PHYTOSTEROLS; IN vivo studies; THERAPEUTICS
- Publication
American Journal of Chinese Medicine, 2018, Vol 46, Issue 5, p1045
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X18500544