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- Title
One-step immortalization of primary human airway epithelial cells capable of oncogenic transformation.
- Authors
Smith, Jordan L.; Liam C. Lee; Read, Abigail; Qiuning Li; Bing Yu; Chih-Shia Lee; Ji Luo
- Abstract
Background: The ability to transform normal human cells into cancer cells with the introduction of defined genetic alterations is a valuable method for understanding the mechanisms of oncogenesis. Easy establishment of immortalized but non-transformed human cells from various tissues would facilitate these genetic analyses. Results: We report here a simple, one-step immortalization method that involves retroviral vector mediated coexpression of the human telomerase protein and a shRNA targeting the CDKN2A gene locus. We demonstrate that this method could successfully immortalize human small airway epithelial cells while maintaining their chromosomal stability. We further showed that these cells retain p53 activity and can be transformed by the KRAS oncogene. Conclusions: Our method simplifies the immortalization process and is broadly applicable for establishing immortalized epithelial cell lines from primary human tissues for cancer research.
- Subjects
CANCER cells; CYCLIN-dependent kinase inhibitor-2A; EPITHELIAL cells
- Publication
Cell & Bioscience, 2016, Vol 6, p1
- ISSN
2045-3701
- Publication type
Article
- DOI
10.1186/s13578-016-0122-6