We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
SARS CoV-2 spike protein-guided exosome isolation facilitates detection of potential miRNA biomarkers in COVID-19 infections.
- Authors
Mimmi, Selena; Zimbo, Anna Maria; Rotundo, Salvatore; Cione, Erika; Nisticò, Nancy; Aloisio, Annamaria; Maisano, Domenico; Tolomeo, Anna Maria; Dattilo, Vincenzo; Lionello, Rosaria; Fioravanti, Antonella; Di Loria, Antonio; Quirino, Angela; Marascio, Nadia; Russo, Alessandro; Trecarichi, Enrico Maria; Matera, Giovanni; Quinto, Ileana; Torti, Carlo; Iaccino, Enrico
- Abstract
Nearly three years into the pandemic, SARS-CoV-2 infections are occurring in vaccinated and naturally infected populations. While humoral and cellular responses in COVID-19 are being characterized, novel immune biomarkers also being identified. Recently, an increase in angiotensin-converting enzyme 2 expressing (aka, ACE2 positive) circulating exosomes (ExoACE2) were identified in the plasma of COVID-19 patients (El-Shennawy et al.). In this pilot study, we describe a method to characterize the exosome-associated microRNA (exo-miRNA) signature in ACE2-positive and ACE2-negative exosomal populations (non-ExoACE2). We performed a sorting protocol using the recombinant biotin-conjugated SARS CoV-2 spike protein containing the receptor binding domain (RBD) on plasma samples from six patients. Following purification, exo-miRNA were characterized for ACE2-positive and ACE2-negative exosome subpopulations by RT-PCR. We identified differential expression of several miRNA. Specifically let-7g-5p and hsa-miR-4454+miR-7975 were upregulated, while hsa-miR-208a-3p and has-miR-323-3p were downregulated in ExoACE2 vs. non-ExoACE2. The SARS CoV-2 spike-protein guided exosome isolation permits isolation of ExoACE2 exosomes. Such purification facilitates detailed characterization of potential biomarkers (e.g. exo-miRNA) for COVID-19 patients. This method could be used for future studies to further the understanding mechanisms of host response against SARS CoV-2.
- Subjects
SARS-CoV-2; COVID-19; CORONAVIRUS spike protein; EXOSOMES; COVID-19 pandemic
- Publication
Clinical Chemistry & Laboratory Medicine, 2023, Vol 61, Issue 8, p1518
- ISSN
1434-6621
- Publication type
Article
- DOI
10.1515/cclm-2022-1286