We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Multi-omics approaches for comprehensive analysis and understanding of the immune response in the miniature pig breed.
- Authors
Arora, Devender; Park, Jong-Eun; Lim, Dajeong; Cho, In-Cheol; Kang, Kyung Soo; Kim, Tae-Hun; Park, Woncheoul
- Abstract
The porcine immune system has an important role in pre-clinical studies together with understanding the biological response mechanisms before entering into clinical trials. The size distribution of the Korean minipig is an important feature that make this breed ideal for biomedical research and safe practice in post clinical studies. The extremely tiny (ET) minipig serves as an excellent model for various biomedical research studies, but the comparatively frail and vulnerable immune response to the environment over its Large (L) size minipig breed leads to additional after born care. To overcome this pitfall, comparative analysis of the genomic regions under selection in the L type breed could provide a better understanding at the molecular level and lead to the development of an enhanced variety of ET type minipig. In this study, we utilized whole genome sequencing (WGS) to identify traces of artificial selection and integrated them with transcriptome data generated from blood samples to find strongly selected and differentially expressed genes of interest. We identified a total of 35 common genes among which 7 were differentially expressed and showed selective sweep in the L type over the ET type minipig breed. The stabilization of these genes were further confirmed using nucleotide diversity analysis, and these genes could serve as potential biomarkers for the development of a better variety of ET type pig breed.
- Subjects
IMMUNE response; SWINE; WHOLE genome sequencing; GENOMICS; SWINE breeds; MEDICAL research
- Publication
PLoS ONE, 2022, Vol 17, Issue 5, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0263035