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- Title
Fast in-vitro screening of FLT3-ITD inhibitors using silkworm-baculovirus protein expression system.
- Authors
Yamamoto, Naoki; Kikuchi, Jiro; Furukawa, Yusuke; Shibayama, Naoya
- Abstract
We report expression and purification of a FLT3 protein with ITD mutation (FLT3-ITD) with a steady tyrosine kinase activity using a silkworm-baculovirus system, and its application as a fast screening system of tyrosine kinase inhibitors. The FLT3-ITD protein was expressed in Bombyx mori L. pupae infected by gene-modified nucleopolyhedrovirus, and was purified as an active state. We performed an inhibition assay using 17 kinase inhibitors, and succeeded in screening two inhibitors for FLT3-ITD. The result has paved the way for screening FLT3-ITD inhibitors in a fast and easy manner, and also for structural studies.
- Subjects
PROTEIN expression; PROTEIN-tyrosine kinase inhibitors; SILKWORMS; PROTEIN-tyrosine kinases; PUPAE; KINASE inhibitors
- Publication
PLoS ONE, 2022, Vol 17, Issue 5, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0261699