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- Title
RAPD Analysis of Romanian Silkworm Genetic Biodiversity (Races X Hybrids).
- Authors
Furdui, Emilia; Märghitaş, Liviu Al.; Dezmirean, Dan; Paşca, Ioan
- Abstract
Genetic differences are followed in the present study between different Bombyx mon genotypes from Romania, with the help of RAPD techniques. DNA extraction was made by using several working protocols: classic (Suzuki et a!., 1972) and 2 rapid ones (extraction kits from Promega: WGDP - Wizard Genomic DNA Purification Kit and WMDP - Wizard Maxwell DNA Purification Kit). 44 primers were used and 34 have generated polymorphic bands. Amplification products resulted by RAPD are different due to the genotype analyzed lines structures, resulting significant differences between strains and hybrids. Bombyx mon genome analysis was initiated having the purpose to generate genetic maps for the characterization of genetic diversity using different marker systems: RFLP, RAPD.
- Subjects
ROMANIA; NUCLEIC acids; GENETIC polymorphisms; GENETIC research; DEOXYRIBOSE; GENETIC transformation; ANTISENSE nucleic acids
- Publication
Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Animal Science & Biotechnologies, 2009, Vol 66, Issue 1/2, p430
- ISSN
1843-5262
- Publication type
Article