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- Title
Application of the NucliSENS easyMAG system for nucleic acid extraction: optimization of DNA extraction for molecular diagnosis of parasitic and fungal diseases.
- Authors
Jeddi, Fakhri; Piarroux, Renaud; Mary, Charles
- Abstract
The article presents the study which examined the effectiveness of using the semi-automated NucliSENS easyMAG system for nucleic acid extraction, with an aim to optimize the DNA extraction for the molecular diagnosis of parasitic and fungal diseases. Also cited are the sample processing variables like optimal cell number range for parasite quantification. The method used to evaluate the results is the quantitative polymerase chain reaction (qPCR).
- Subjects
NUCLEIC acid isolation methods; GENETIC techniques; PARASITIC diseases; MYCOSES; POLYMERASE chain reaction
- Publication
Parasite (1252607X), 2013, Vol 20, p1
- ISSN
1252-607X
- Publication type
Article
- DOI
10.1051/parasite/2013051