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- Title
Sarcocyst Quantification and Viability: Freezing Treatment as an Alternative to Carcass Condemnation.
- Authors
Rodrigues de Freitas, Bibiana; Roman, Isac Junior; Reis, Bibiana Rossi; de Souza Rodrigues, Fernando; Braünig, Patrícia; Cargnelutti, Juliana Felipetto; Cunha, Rodrigo Casquero; Vogel, Fernanda Silveira Flôres
- Abstract
Purpose: The inspection of animal products is important for controlling parasitic zoonoses. Some processes that guarantee food safety to consumers such as carcass condemnation cause economic losses. This study aimed to detect Sarcocystis cysts in cattle hearts obtained from slaughterhouses and to evaluate sarcocyst viability after freezing treatment. Methods: When myocardial tissues were minced and subjected to fresh examination, sarcocysts were observed in all analyzed tissues resulting in 21.73 cysts/g of tissue. Sarcocyst viability was verified after tissue freezing at 35 ± 2 °C and – 20 ± 2 °C for 0–12 h. After freezing, the tissues were minced, and sarcocysts were collected and stained with Tripan Blue. In addition, cysts were mechanically disrupted to check bradyzoite viability. Results: Cysts and bradyzoites were unviable at – 35 °C for ≥ 3 h and − 20 °C for ≥ 8 h. Conclusion: These results suggest freezing treatment as an alternative to condemnation of cattle carcasses contaminated with Sarcocystis spp. Similar studies using freezing treatment with other animals infected by Sarcocystis must be conducted.
- Subjects
CATTLE carcasses; ANIMAL products; ANIMAL welfare; SARCOCYSTIS; FROZEN semen; FOOD safety; FREEZING
- Publication
Acta Parasitologica, 2023, Vol 68, Issue 1, p277
- ISSN
1230-2821
- Publication type
Article
- DOI
10.1007/s11686-022-00656-9