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- Title
Optimization of fermentation process of Cordyceps militaris and antitumor activities of polysaccharides in vitro.
- Authors
Shuang Yang; Lu Jin; Xiaodong Ren; Jiahui Lu; Qingfan Meng
- Abstract
The influence of medium composition and cultural conditions on simultaneous yield of mycelia, intracellular polysaccharide, adenosine, and mannitol by Cordyceps militaris CGMCC 2909 was investigated with desirability functions in this study. An optimization strategy based on the desirability function approach, together with response surface methodology (RSM) has been used to optimize medium composition, and the optimal medium was obtained via the desirability as follows: yeast extract 10.33 g/L, sucrose 27.24 g/L, KH2PO4 5.60 g/L and the optimal culture conditions are initial pH 6, 25°C, rotation speed 150 r/minute, inoculum size 4%(v/v), and medium capacity 40 mL/250 mL. Under these conditions, the yield of mycelia, intracellular polysaccharide, adenosine and mannitol reached 12.19 g/L, 0.6 g/L, 61.84 mg/L, and 1.38 g/L, respectively, and the D value was 0.77. Furthermore, the polysaccharides showed significant antitumor activities against HeLa and HepG2 in vitro in a dose-dependent manner in 72 hours. At a concentration of 1000 mg/mL, the inhibition rate of polysaccharides was 92.38% and 98.79%. The IC50 for HeLa and HepG2 were 70.91 µg/mL and 97.63 µg/mL, respectively.
- Subjects
ADENOSINES; ANALYSIS of variance; ANTINEOPLASTIC agents; CELL lines; FERMENTATION; FUNGI; HIGH performance liquid chromatography; CHINESE medicine; POLYSACCHARIDES; T-test (Statistics); MANNITOL; DATA analysis software; IN vitro studies
- Publication
Journal of Food & Drug Analysis, 2014, Vol 22, Issue 4, p468
- ISSN
1021-9498
- Publication type
Article
- DOI
10.1016/j.jfda.2014.01.028