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- Title
RAPID IDENTIFICATION FOR SEROTYPING OF AFRICAN SWINE FEVER VIRUS BASED ON THE SHORT FRAGMENT OF THE EP402R GENE ENCODING FOR CD2-LIKE PROTEIN.
- Authors
Ha Thi Thanh, TRAN; Anh Duc, TRUONG; Duc Viet, LY; Tuan Van, HOANG; Chinh Thi, NGUYEN; Nhu Thi, CHU; Huyen Thi, NGUYEN; Hoang Vu, DANG
- Abstract
The first confirmed case of African swine fever (ASF) in Vietnam was officially reported in February 2019. To date, the ASF virus (ASFV) has been detected in all 63 cities/provinces in Vietnam. In order to get a better understanding of the potential role of the EP402R gene in a grouping of ASFV serotypes, thirty ASFV sequences of EP402R genes (accession numbers: MN711757-86) from North Central Coast of Vietnam and 68 well-known references of serotype groups from previous studies were further analyzed. Interestingly, we found that a short fragment of 90 nucleotides was very typical for 8 serological groups of ASFVs. A primer set was designed to amplicon the short fragment of 90 nucleotides using the Primer3 program to establish a simplified method for the serotyping of ASFV. Our results indicated that phylogenetic analysis of the short fragment (90 nucleotides) of the EP402R gene is a very specific and useful method for ASFV serotyping when compared to the previous method using a long fragment (816 nucleotides) of this gene and well-known serotype references based on haemadsorption inhibition (HAI) assay.
- Subjects
VIETNAM; AFRICAN swine fever virus; AFRICAN swine fever; CLASSICAL swine fever; SEROTYPING; GENES
- Publication
Acta Veterinaria, 2021, Vol 71, Issue 1, p98
- ISSN
0567-8315
- Publication type
Article
- DOI
10.2478/acve-2021-0007