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- Title
A Medium-Term, Rapid Rat Bioassay Model for the Detection of Carcinogenic Potential of Chemicals.
- Authors
Tsuda, Hiroyuki; Futakuchi, Mitsuru; Fukamachi, Katsumi; Shirai, Tomoyuki; Imaida, Katsumi; Fukushima, Shoji; Tatematsu, Masae; Furukawa, Fumio; Tamano, Seiko; Ito, Nobuyuki
- Abstract
The Ito Liver Model and the Ito Multi-organ Model are used in conjunction and constitute an efficient and rapid bioassay for the identification of both genotoxic and nongenotoxic carcinogenic chemicals. The Ito Liver Model is an 8-week bioassay system that uses the number and size of foci of hepatocytes positive for glutathione S-transferase placental form (GST-P) as the end-point marker. One hundred fifty-nine compounds were tested using the Ito Liver Model: 61 of 66 hepatocarcinogens tested positive, and 10 of 43 nonliver carcinogens were also positive. The false-positive detection of noncarcinogens was low; a single false-positive result was obtained from the 50 noncarcinogens tested. Since more than half of all known carcinogens are hepatocarcinogens in rodents, the initial 8-week bioassay is able to detect most carcinogens. The Ito Multi-organ Model is a 28-week bioassay system for the detection of carcinogens that were not identified by the Ito Liver Model. Results are evaluated by preneoplastic and neoplastic lesions in major organs. Forty-four compounds were tested using the Ito Multi-organ Model: 17 out of 17 liver carcinogens were positive, and 19 out of 22 (86%) nonliver carcinogens were positive. None of the five noncarcinogens tested positive.
- Subjects
BIOLOGICAL assay; RATS; ANIMAL models in research; CHEMICALS; CARCINOGENS; GLUTATHIONE transferase
- Publication
Toxicologic Pathology, 2010, Vol 38, Issue 1, p182
- ISSN
0192-6233
- Publication type
Article
- DOI
10.1177/0192623309356451