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- Title
Reduced Expression of PD-1 in Circulating CD4+ and CD8+ Tregs Is an Early Feature of RRMS.
- Authors
Machcińska, Maja; Kierasińska, Magdalena; Michniowska, Martyna; Maruszewska-Cheruiyot, Marta; Szewczak, Ludmiła; Rola, Rafał; Karlińska, Anna; Stear, Michael; Donskow-Łysoniewska, Katarzyna
- Abstract
Altered regulatory T cell (Treg) function could contribute to MS. The expression of activating and inhibitory receptors influences the activity of Tregs. Our aim was to investigate T cell phenotypes in relapsing–remitting MS (RRMS) patients at an early phase of the disease. We examined the influence of demographic parameters on the distribution of CD4+ and CD8+ T cell subclasses by generalized linear modeling. We also studied the expression of the following markers—CTLA-4, GITR, PD-1, FoxP3, Helios, CD28, CD62L, CD103—on T cell subsets from peripheral blood with a 14-color flow cytometry panel. We used an antibody array to define the profiles of 34 Th1/Th2/Th17 cytokines in the serum. Expression of PD-1 and GITR on CD4+ and CD8+ Tregs was decreased in RRMS patients. The proinflammatory factors IFN-γ, IL-17, IL-17F, TGFβ-1, TGFβ-3, IL-1SRII, IL-12 p40, sgp130, IL-6sR were significantly increased in RRMS patients. Therefore, a deficiency of PD-1 and GITR immune checkpoints on CD4+ and CD8+ Tregs is a feature of RRMS and might underlie impaired T cell control.
- Subjects
PROGRAMMED cell death 1 receptors; REGULATORY T cells; CD8 antigen; IMMUNE checkpoint proteins; CD4 antigen
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 6, p3185
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23063185