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- Title
Template-Assisted Plasmonic Nanogap Shells for Highly Enhanced Detection of Cancer Biomarkers.
- Authors
Kang, Homan; Jeong, Sinyoung; Yang, Jin-Kyoung; Jo, Ahla; Lee, Hyunmi; Heo, Eun Hae; Jeong, Dae Hong; Jun, Bong-Hyun; Chang, Hyejin; Lee, Yoon-Sik; Baldi, Alfonso
- Abstract
We present a template-assisted method for synthesizing nanogap shell structures for biomolecular detections based on surface-enhanced Raman scattering. The interior nanogap-containing a silver shell structure, referred to as a silver nanogap shell (Ag NGS), was fabricated on silver nanoparticles (Ag NPs)-coated silica, by adsorbing small aromatic thiol molecules on the Ag NPs. The Ag NGSs showed a high enhancement factor and good signal uniformity, using 785-nm excitation. We performed in vitro immunoassays using a prostate-specific antigen as a model cancer biomarker with a detection limit of 2 pg/mL. To demonstrate the versatility of Ag NGS nanoprobes, extracellular duplex surface-enhanced Raman scattering (SERS) imaging was also performed to evaluate the co-expression of cancer biomarkers, human epidermal growth factor-2 (HER2) and epidermal growth factor receptor (EGFR), in a non-small cell lung cancer cell line (H522). Developing highly sensitive Ag NGS nanoprobes that enable multiplex biomolecular detection and imaging can open up new possibilities for point-of-care diagnostics and provide appropriate treatment options and prognosis.
- Subjects
SERS spectroscopy; EPIDERMAL growth factor receptors; NON-small-cell lung carcinoma; PROSTATE-specific antigen; BIOMARKERS
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 4, p1752
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms22041752