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- Title
Genome-Wide Detection of Key Genes and Epigenetic Markers for Chicken Fatty Liver.
- Authors
Tan, Xiaodong; Liu, Ranran; Xing, Siyuan; Zhang, Yonghong; Li, Qinghe; Zheng, Maiqing; Zhao, Guiping; Wen, Jie
- Abstract
Chickens are one of the most important sources of meat worldwide, and the occurrence of fatty liver syndrome (FLS) is closely related to production efficiency. However, the potential mechanism of FLS remains poorly understood. An integrated analysis of data from whole-genome bisulfite sequencing and long noncoding RNA (lncRNA) sequencing was conducted. A total of 1177 differentially expressed genes (DEGs) and 1442 differentially methylated genes (DMGs) were found. There were 72% of 83 lipid- and glucose-related genes upregulated; 81% of 150 immune-related genes were downregulated in fatty livers. Part of those genes was within differentially methylated regions (DMRs). Besides, sixty-seven lncRNAs were identified differentially expressed and divided into 13 clusters based on their expression pattern. Some lipid- and glucose-related lncRNAs (e.g., LNC_006756, LNC_012355, and LNC_005024) and immune-related lncRNAs (e.g., LNC_010111, LNC_010862, and LNC_001272) were found through a co-expression network and functional annotation. From the expression and epigenetic profiles, 23 target genes (e.g., HAO1, ABCD3, and BLMH) were found to be hub genes that were regulated by both methylation and lncRNAs. We have provided comprehensive epigenetic and transcriptomic profiles on FLS in chicken, and the identification of key genes and epigenetic markers will expand our understanding of the molecular mechanism of chicken FLS.
- Subjects
FATTY liver; EPIGENETICS; CHICKENS; NON-coding RNA; GENES; LINCRNA; CHICKEN diseases
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 5, p1800
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21051800