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- Title
Isolation and Characterization of Microsatellite Markers in Brown Planthopper (Nilaparvata lugens Stål).
- Authors
Shengli Jing; Xi Zhou; Hangjin Yu; Bingfang Liu; Chunxiao Zhang; Shuzhen Wang; Xinxin Peng; Lili Zhu; Yi Ding; Guangcun He
- Abstract
Brown planthopper (Nilaparvata lugens Stål) (Homoptera: Delphacidae) is an economically important pest on rice. In this study, 30 polymorphic microsatellite markers were developed from N. lugens genomic libraries using the method of Fast Isolation by AFLP of Sequence Containing Repeats (FIASCO). Polymorphism of each locus was detected in 48 individuals from two natural populations. These microsatellite loci revealed 2 to 18 alleles, and the expected and observed heterozygosities ranged from 0.042 to 0.937 and from 0.042 to 0.958, respectively. These markers will be useful for the future study of this agricultural pest in population genetics and molecular genetics.
- Subjects
ALLELES; MICROSATELLITE repeats; NILAPARVATA lugens; RICE genetics; RICE disease &; pest resistance; BIOMARKERS; GENETIC polymorphisms
- Publication
International Journal of Molecular Sciences, 2012, Vol 13, Issue 8, p9527
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms13089527