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- Title
Enzyme biosensor based on the immobilization of HRP on SiO/BSA/Au composite nanoparticles.
- Authors
Yuan, Shirong; Yuan, Ruo; Chai, Yaqin; Zhuo, Ying; Yang, Xia; Yuan, Yali
- Abstract
In this work, an enzyme biosensor based on the immobilization of horseradish peroxidase (HRP) on SiO/BSA/Au/thionine/nafion-modified gold electrode was fabricated successfully. Firstly, nafion was dropped on the surface of the gold electrode to form a nafion film followed by chemisorption of thionine (Thi) as an electron mediator via the ion-exchange interaction between the Thi and nafion. Subsequently, the SiO/BSA/Au composite nanoparticles were assembled onto Thi film through the covalent bounding with the amino groups of Thi. Finally, HRP was immobilized on the SiO/BSA/Au composite nanoparticles due to the covalent conjugation to construct an enzyme biosensor. The surface topographies of the SiO/BSA/Au composite nanoparticles were investigated by using scanning electronic microscopy. The stepwise self-assemble procedure of the biosensor was further characterized by means of cyclic voltammetry and chronoamperometry. The enzyme biosensor showed high sensitivity, good stability and selectivity, a wide linear response to hydrogen peroxide (HO) in the range of 8.0 × 10 ∼ 3.72 × 10 mol/L, with a detection limit of 2.0 × 10 mol/L. The Michaelies-Menten constant $$ K_M^{app} $$ value was estimated to be 2.3 mM.
- Subjects
ENZYMES; BIOSENSORS; HORSERADISH; PEROXIDASE; NANOPARTICLES; SCANNING electron microscopy; ELECTRONS; IMMOBILIZED enzymes
- Publication
Applied Biochemistry & Biotechnology, 2010, Vol 162, Issue 8, p2189
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-010-8993-1