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- Title
C-terminal cleavage of the LH1 α-polypeptide in the Sr-cultured Thermochromatium tepidum.
- Authors
Kimura, Yukihiro; Kawakami, Tomoaki; Arikawa, Teruhisa; Li, Yong; Yu, Long-Jiang; Ohno, Takashi; Madigan, Michael T.; Wang-Otomo, Zheng-Yu
- Abstract
The light-harvesting 1 reaction center (LH1-RC) complex in the thermophilic purple sulfur bacterium Thermochromatium ( Tch.) tepidum binds Ca ions as cofactors, and Ca-binding is largely involved in its characteristic Q absorption at 915 nm and enhanced thermostability. Ca can be biosynthetically replaced by Sr in growing cultures of Tch. tepidum. However, the resulting Sr-substituted LH1-RC complexes in such cells do not display the absorption maximum and thermostability of those from Ca-grown cells, signaling that inherent structural differences exist in the LH1 complexes between the Ca- and Sr-cultured cells. In this study, we examined the effects of the biosynthetic Sr-substitution and limited proteolysis on the spectral properties and thermostability of the Tch. tepidum LH1-RC complex. Preferential truncation of two consecutive, positively charged Lys residues at the C-terminus of the LH1 α-polypeptide was observed for the Sr-cultured cells. A proportion of the truncated LH1 α-polypeptide increased during repeated subculturing in the Sr-substituted medium. This result suggests that the truncation is a biochemical adaptation to reduce the electrostatic interactions and/or steric repulsion at the C-terminus when Sr substitutes for Ca in the LH1 complex. Limited proteolysis of the native Ca-LH1 complex with lysyl protease revealed selective truncations at the Lys residues in both C- and N-terminal extensions of the α- and β-polypeptides. The spectral properties and thermostability of the partially digested native LH1-RC complexes were similar to those of the biosynthetically Sr-substituted LH1-RC complexes in their Ca-bound forms. Based on these findings, we propose that the C-terminal domain of the LH1 α-polypeptide plays important roles in retaining proper structure and function of the LH1-RC complex in Tch. tepidum.
- Subjects
CHROMATIACEAE; LIGHT-harvesting complex (Photosynthesis); C-terminal residues; THERMOPHILIC bacteria; PHOTOSYNTHETIC reaction centers; BIOSYNTHESIS; CALCIUM ions; STRONTIUM; BACTERIA
- Publication
Photosynthesis Research, 2018, Vol 135, Issue 1-3, p23
- ISSN
0166-8595
- Publication type
Article
- DOI
10.1007/s11120-017-0393-8