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- Title
Chip-calorimetric monitoring of biofilm eradication with bacteriophages reveals an unexpected infection-related heat profile.
- Authors
Morais, Frida; Buchholz, Friederike; Hartmann, Tom; Lerchner, Johannes; Neu, Thomas; Kiesel, Bärbel; Harms, Hauke; Maskow, Thomas
- Abstract
Bacteriophages or phage-derived biological structures are a promising alternative to the application of antibiotics to eradicate biofilms. These countermeasures are highly cell specific. For a better understanding of the sequence of the underlying processes (attachment, infection, multiplication, phage release), for optimization of phage applications, or simply for screening of suitable phages or phage-derived enzymes, real-time monitoring devices are urgently required. Calorimetry is promising because it is non-invasive and quantitatively connected to the metabolic fluxes. Chip-calorimetry provides real-time information about biofilm eradication by phages. This was confirmed by comparison with reference analyses (i.e., confocal laser scanning microscopy, colony plate counts, or phage titre determination). Furthermore, chip-calorimetry provides additional information which was not captured by the reference methods such as the enhanced cell-specific heat production caused by the infection process and a residual activity of seemingly persistent bacteria.
- Subjects
BACTERIOPHAGES; CALORIMETRY; BIOFILMS; HEAT production (Biology); ANTIBIOTICS; ENZYMES; BACTERIAL cells
- Publication
Journal of Thermal Analysis & Calorimetry, 2014, Vol 115, Issue 3, p2203
- ISSN
1388-6150
- Publication type
Article
- DOI
10.1007/s10973-013-3494-4