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- Title
It Takes Two to Tango: Combining Conventional Culture With Molecular Diagnostics Enhances Accuracy of Streptococcus pneumoniae Detection and Pneumococcal Serogroup/Serotype Determination in Carriage.
- Authors
Miellet, Willem R.; van Veldhuizen, Janieke; Litt, David; Mariman, Rob; Wijmenga-Monsuur, Alienke J.; Badoux, Paul; Nieuwenhuijsen, Tessa; Thombre, Rebecca; Mayet, Sanaa; Eletu, Seyi; Sheppard, Carmen; van Houten, Marianne Alice; Rots, Nynke Y.; Miller, Elizabeth; Fry, Norman K.; Sanders, Elisabeth A. M.; Trzciński, Krzysztof
- Abstract
For serogroup-specific qPCR assays a result was considered congruent when a serogroup was detected in qPCR that matched the serogroup of the serotype detected by culture. Sample was classified as positive for a serotype/serogroup with molecular method when the signal detected by qPCR for I piaB i ( I X i -axis) and serotype/serogroup ( I Y i -axis) were both below the SP ROCd sp C I SB q sb i cut-off threshold of 30 C I SB q sb i . In the panel depicting qPCR results for serogroup 6 all symbols represent samples identified as positive for serotypes 6C or 6D except for red circles depicting two samples identified as positive for serotype 6A or 6B, both positive for serotype 6A by culture. In the panel depicting qPCR results for serogroup 22 all symbols represent positivity for serotype 22F. None of the samples generated any signal in qPCRs targeting serotype 1, serotype 23F and serogroup 18.
- Subjects
STREPTOCOCCUS pneumoniae; PNEUMOCOCCAL pneumonia; PNEUMOCOCCAL meningitis; MOLECULAR diagnosis
- Publication
Frontiers in Microbiology, 2022, Vol 13, p1
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2022.859736