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- Title
Nucleoid organization in the radioresistant bacterium D einococcus radiodurans.
- Authors
Passot, Fanny Marie; Nguyen, Hong Ha; Dard‐Dascot, Cloelia; Thermes, Claude; Servant, Pascale; Espéli, Olivier; Sommer, Suzanne
- Abstract
Processes favoring the exceptional resistance to genotoxic stress of D einococcus radiodurans are not yet completely characterized. It was postulated that its nucleoid and chromosome(s) organization could participate in the DNA double strand break repair process. Here, we investigated the organization of chromosome 1 by localization of three chromosomal loci including oriC, Ter and a locus located in its left arm. For this purpose, we used a ParB- parS system to visualize the position of the loci before and after exposure to γ-rays. By comparing the number of fluorescent foci with the number of copies of the studied loci present in the cells measured by quantitative polymerase chain reaction ( qPCR), we demonstrated that the 4-10 copies of chromosome 1 per cell are dispersed within the nucleoid before irradiation, indicating that the chromosome copies are not prealigned. Chromosome segregation is progressive but not co-ordinated, allowing each locus to be paired with its sister during part of the cell cycle. After irradiation, the nucleoid organization is modified, involving a transient alignment of the loci in the late stage of DNA repair and a delay of segregation of the Ter locus. We discuss how these events can influence DNA double strand break repair.
- Subjects
GENETIC engineering of Deinococcus radiodurans; NUCLEOIDS; BACTERIAL DNA; POLYMERASE chain reaction; DNA repair
- Publication
Molecular Microbiology, 2015, Vol 97, Issue 4, p759
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/mmi.13064