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- Title
Inhibition of malignant phenotypes of human osteosarcoma cells by a gene silencer, a pyrrole–imidazole polyamide, which targets an E‐box motif.
- Authors
Taniguchi, Masashi; Fujiwara, Kyoko; Nakai, Yuji; Ozaki, Toshinori; Koshikawa, Nobuko; Toshio, Kojima; Kataba, Motoaki; Oguni, Asako; Matsuda, Hiroyuki; Yoshida, Yukihiro; Tokuhashi, Yasuaki; Fukuda, Noboru; Ueno, Takahiro; Soma, Masayoshi; Nagase, Hiroki
- Abstract
Gene amplification and/or overexpression of the transcription factor c‐MYC, which binds to the E‐box sequence (5′‐CACGTG‐3′), has been observed in many human tumors. In this study, we have designed 5 pyrrole–imidazole (PI) polyamides recognizing E‐box, and found that, among them, Myc‐6 significantly suppresses malignant phenotypes of human osteosarcoma MG63 cells both in vitro and in vivo. Intriguingly, knockdown of the putative Myc‐6 target MALAT1 encoding long noncoding RNA remarkably impaired cell growth of MG63 cells. Collectively, our present findings strongly suggest that Myc‐6 exerts its tumor‐suppressive ability at least in part through the specific down‐regulation of MALAT1.
- Subjects
GENE amplification; GENETIC overexpression; TRANSCRIPTION factors; PYRROLES; POLYAMIDES; NON-coding RNA
- Publication
FEBS Open Bio, 2014, Vol 4, Issue 1, p328
- ISSN
2211-5463
- Publication type
Article
- DOI
10.1016/j.fob.2014.03.004