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- Title
The effect of different methods of neurorrhaphy on the supplied muscle in a rat model of nerve injury: a comparative histological and immunohistochemical study.
- Authors
Mostafa, Hany K.; Gad, Ahmed M.; Sadek, Bassem M.; Sadek, Eman Y.; Sherif, Mahmoud M.
- Abstract
Background Several methods of reconstruction exist for nerve injuries with a significant nerve gap. The most important of them is end-to-side neurorrhaphy (ETSN). It is the technique of creating a neurorrhaphy between the distal end of an injured nerve and the side of a healthy nerve. Aim The aim of this study was to compare the efficacy of motor (peroneal) nerve ETSN either with mixed nerve or motor nerve on the cranial tibial muscle (CTM) supplied by the peroneal nerve. Materials and methods Fifty Wistar adult male albino rats with an average weight of 250 g were divided into five groups (10 animals each). Group I was the control group. In group II, the CTM was denervated by cutting the peroneal nerve, and in group III, the peroneal nerve was cut and an end-to-end neurorrhaphy (primary neurorrhaphy) was performed. In group IV the peroneal nerve was cut and an ETSN between the peroneal nerve and motor nerve was created, and in group V the peroneal nerve was cut and an ETSN between the peroneal nerve and mixed nerve was created. After 120 days the CTM specimens were dissected out and processed for H&E and immunostaining for desmin protein. Results In histological study, muscle fibers of group II showed a significant decrease in mean diameter and an increased connective tissue spaces. Moreover, immunostaining for desmin was faint. The best experimental groups were group III and group V, which showed findings similar to that of the control. Group IV showed variably sized fibers, centrally placed nuclei, and faint immunostaining for desmin. Moreover, morphometric and statistical results confirmed the microscopic findings. Conclusion The results of the present study suggested that ETSN with mixed nerve has great advantage over ETSN with motor nerve on supplied muscle.
- Publication
Egyptian Journal of Histology, 2016, Vol 39, Issue 1, p50
- ISSN
1110-0559
- Publication type
Article
- DOI
10.1097/01.EHX.0000481743.90311.73