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- Title
Tc99m Kullanımının Kütle Spektrometri Tekniğiyle Ölçülen Takrolimus, Everolimus, Sirolimus ve Siklosporin A Düzeylerine Etkisinin Değerlendirilmesi.
- Authors
YILMAZTEKİN, Mehmet Zeki; GÖNEL, Ataman; GÜZELÇİÇEK, Ahmet; KOYUNCU, İsmail; BAYRAKTAR, Nihayet
- Abstract
Background: To investigate the effect of Tc99m exposure on tacrolimus, everolimus, sirolimus and cyclosporin A levels measured by blood LC / MSMS used in scintigraphic imaging techniques. Materials and Methods: HPLC solvents, reagents (I Jasem, Turkey), calibrator and control solutions for the study were employed. The Tc99m preparation was used for the interference study. Immunosuppressant measurement was performed by Shimadzu 8045 triple quadrupole mass spectrometry (MS / MS) (Shimadzu, Japan), which was integrated with Shimadzu Nexera X2 ultra high-performance liquid chromatography (UHPLC). Device data was calculated with Shimadzu Software. For sample preparation, 500 microliters (LL) of control solution was transferred to the centrifuge tube. 25 uL of internal standard was added and mixed with vortex for 5 seconds. All procedures for interference study were repeated for level1, level2 and level3 control solutions and performed by LC/MS-MS. Interference protocol was repeated by adding 50uL of distilled water to each level of control. Results: Bias was detected in the range of 6.28% to 47.21% in all sirolimus measurements. Tacrolimus levels deviated from the target value in the range of 21.81% to 44.62%. For cyclosporine A levels, bias was calculated between 9,30% - 43,16%. The least deviation was observed in Sirolimus level 3 with 6.28% and the maximum deviation was observed in sirolimus level 1. Conclusion: The Tc99m radionuclide used in scintigraphic imaging has the potential to affect immunosuppressant levels measured by LC / MS-MS devices. Health service staffs making sampling should be informed about this and physicians should consider the interference posibility in suspicious immunosuppressant concentrations.
- Subjects
JAPAN; COMPUTER software; TACROLIMUS; IMMUNOSUPPRESSIVE agents; LIQUID chromatography; MASS spectrometry; RADIONUCLIDE imaging; TECHNETIUM; RAPAMYCIN; CYCLOSPORINS; EVEROLIMUS; PHARMACODYNAMICS
- Publication
Journal of Harran University Medical Faculty / Harran Üniversitesi Tıp Fakültesi Dergisi, 2019, Vol 16, Issue 3, p488
- ISSN
1304-9623
- Publication type
Article
- DOI
10.35440/hutfd.650152