We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Development and Validation of a Transcriptome-Based Simple Sequence Repeats Markers in Coilia nasus.
- Authors
Fukuan Du; Yongkai Tang; Juhua Yu; Shengyan Su; Fan Yu; Jianlin Li; Hongxia Li; Meiyao Wang; Pao Xu
- Abstract
Estuarine tapertail anchovy (Coilia nasus) is a commercially important species. However, eco-environment deterioration and overfishing have almost caused extinction of the species in the Yangtze River. Therefore, it is very urgent to perform genetic research on C. nasus to protect the wild population. In this study, we sequenced the brain transcriptome of C. nasus using Illumina Hiseq 4000 platform. We obtained 123,764 unigenes with an average length of 1092 bp and a total of 115,169 putative Simple Sequence Repeats (SSRs). Among them, 37 SSRs were selected for the validation experiments. All of the loci were found to be polymorphic and showed bi-allelic in 60 individuals of C. nasus. These SSR markers should not only be useful for population conservation, but also for construction of genetic linkage map and economic performance improvement of C. nasus.
- Subjects
MICROSATELLITE repeats; BIOLOGICAL extinction; FLOWERING of plants; GENE mapping
- Publication
Pakistan Journal of Zoology, 2019, Vol 51, Issue 4, p1335
- ISSN
0030-9923
- Publication type
Article
- DOI
10.17582/journal.pjz/2019.51.4.1335.1341