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- Title
Chukrasia tabularis limonoid plays anti-inflammatory role by regulating NF-κB signaling pathway in lipopolysaccharide-induced macrophages.
- Authors
Jinhuang Shen; Fan Cao; Zhiyong Huang; Xinhua Ma; Nana Yang; Haitao Zhang; Yonghong Zhang; Zhiqiang Zhang
- Abstract
Background: Chukrasia tabularisis, a well-known tropical tree native to southeastern China, has anti-inflammatory and antioxidant activities, and contains large amounts of limonoids and triterpenoids. Objective: The aim of this study was to investigate the potential anti-inflammatory activity of limonoids from C. tabularis on lipopolysaccharide (LPS)-mediated RAW264.7 cells. Methods and results: Using a bioassay-guided approach, the chemical fraction with high anti-inflammatory activity was found and its chemical constituents were investigated. Phytochemical studies on active extracts resulted in the separation of three novel phragmalin limonoids (1-3), together with two known limonoids (4-5) and 11 tirucallane triterpenes (6-16). The activity of these isolated compounds in the production of nitric oxide (NO) on LPS-reated macrophages was evaluated. Limonoid 2 indicated significant anti-inflammatory activities with IC50 value of 4.58 µM. Limonoid 2 notably inhibited the production of NO, interleukin- 6 and tumor necrosis factor-α on macrophage. Signal transduction and activation of STAT and NF-κB activators were effectively blocked by limonoid 2. Conclusions: These results indicate that limonoid 2 has an anti-inflammatory effect by the inhibiting JAK2/STAT3, iNOS/eNOS, and NF-κB signaling pathways and regulating inflammatory mediators.
- Subjects
LIPOPOLYSACCHARIDES; STAT proteins; NF-kappa B; MACROPHAGES; HYDROCARBONS; CELLULAR signal transduction; JANUS kinases; PHYTOCHEMICALS; FRUIT; RESEARCH funding; MOLECULAR structure; INFLAMMATORY mediators
- Publication
Food & Nutrition Research, 2023, Vol 67, p1
- ISSN
1654-6628
- Publication type
Article
- DOI
10.29219/fnr.v67.9383