We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Interinstitutional variability in DNA flow cytometric analysis of tumors. The National Cancer Institute's Flow Cytometry Network Experience.
- Authors
Coon, John S.; Deitch, Arline D.; De White, Ralph W. Vere; Koss, Leopold G.; Melamed, Myron R.; Reeder, Jay E.; Weinstein, Ronald S.; Wheeless, Leon L.; Wersto, Robert P.; Coon, J S; Deitch, A D; de Vere White, R W; Koss, L G; Melamed, M R; Reeder, J E; Weinstein, R S; Wersto, R P; Wheeless, L L
- Abstract
Flow cytometric DNA analysis of human urinary bladder specimens may be clinically useful for prognosis in transitional cell (urothelial) carcinoma and for detecting recurrence after treatment. However, many important methodological differences exist among institutions which have described this technique, and it has not previously been shown that data from different institutions are comparable. The National Cancer Institute has created a Flow Cytometry Network to address the need for technology assessment of flow cytometry. This report describes the independent flow cytometric analysis and interpretation of "unknown" paraffin-embedded bladder tumor specimens by the five Network institutions. Although important differences in method existed among the institutions, substantial agreement was achieved in actual data generated and their interpretation. This suggests that a consensus regarding acceptable laboratory performance of this technique could be reached, which should facilitate its more widespread clinical implementation.
- Publication
Cancer (0008543X), 1988, Vol 61, Issue 1, p126
- ISSN
0008-543X
- Publication type
journal article
- DOI
10.1002/1097-0142(19880101)61:1<126::AID-CNCR2820610122>3.0.CO;2-5