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- Title
The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif.
- Authors
Gang Dong; Hutagalung, Alex H.; Chunmei Fu; Novick, Peter; Reinisch, Karin M.
- Abstract
The exocyst is a large complex that is required for tethering vesicles at the final stages of the exocytic pathway in all eukaryotes. Here we present the structures of the Exo70p subunit of this complex and of the C-terminal domains of Exo84p, at 2.0-Å and 2.85-Å resolution, respectively. Exo70p forms a 160-Å-long rod with a novel fold composed of contiguous α-helical bundles. The Exo84p C terminus also forms a long rod (80 Å), which unexpectedly has the same fold as the Exo70p N terminus. Our structural results and our experimental observations concerning the interaction between Exo70p and other exocyst subunits or Rho3p GTPase are consistent with an architecture wherein exocyst subunits are composed of mostly helical modules strung together into long rods.
- Subjects
EXOCYTOSIS; CELL physiology; GUANOSINE triphosphatase; GTPASE-activating protein; PROTEIN folding; CELL membranes
- Publication
Nature Structural & Molecular Biology, 2005, Vol 12, Issue 12, p1094
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb1017