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- Title
Expression of Neuro D1 in Human Normal Pituitaries and Pituitary Adenomas.
- Authors
Oyama, Kenichi; Sanno, Naoko; Teramoto, Akira; Osamura, R. Yoshiyuki
- Abstract
Neuro D1 is a basic helix-loop-helix transcription factor expressed in the endocrine cells of pancreas and in a subset of neurons as they undergo terminal differentiation. In the adult pituitary gland, Neuro D1 is expressed in corticotroph cells and contributes to the corticotroph-specific proopiomelanocortin (POMC) transcription by interacting with Pituitary homeobox 1 (Ptx 1) transcription factor. In the present study, we investigated the expression of Neuro D1 in human normal pituitaries and different types of human pituitary adenomas using the RT-PCR and immunohistochemical techniques. Using RT-PCR, Neuro D1 mRNA was found to be expressed in ACTH-secreting adenomas (n = 3) and 6 of 8 nonfunctioning adenomas. On the other hand, GHsecreting adenomas (n = 5) and PRL-secreting adenomas (n = 3) were completely negative for Neuro D1 mRNA. Immunohistochemically, Neuro D1 was expressed in all ACTH-secreting adenomas (n = 10), and in 14 of 20 nonfunctioning adenomas. In contrast, 3 of 10 PRL-secreting adenomas and 2 of 10 GH-secreting adenomas showed positive Neuro D1 staining in the nuclei. The above results suggest that Neuro D1 contribute to the functional expression and the differentiation of ACTH-secreting adenomas. It also appears from our study that Neuro D1 might play a role in the differentiation of nonfunctioning adenomas, the mechanism of which remains to be further investigated. This is the first study on Neuro D1 in case of human pituitary adenomas.
- Subjects
PUBLIC health; PITUITARY gland; PROTEINS; BIOMOLECULES; ORGANIC compounds; MEDICAL care
- Publication
Modern Pathology, 2001, Vol 14, Issue 9, p892
- ISSN
0893-3952
- Publication type
Article
- DOI
10.1038/modpathol.3880408