We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The evolution of two homologues of the core protein VP6 of epizootic haemorrhagic disease virus (EHDV), which correspond to the geographical origin of the virus.
- Authors
S. Maan; G. Sutton; H. Attoui; P. Mertens
- Abstract
Abstract  Epizootic haemorrhagic disease virus is a 10-segmented, double-stranded RNA virus. When these ten segments of dsRNA are run on 1% agarose, eastern (Australia, Japan) and western (North America, Africa, Middle-East) strains of the virus can be separated phenotypically based on the migration of genome segments 7â9. In western strains, segments 7â9 are roughly the same size and co-migrate as a single RNA band. In eastern strains, segment 9 is smaller, so while segments 7 and 8 co-migrate, the segment 9 RNA runs faster than its western homologue. Translation experiments demonstrated that these two segment 9 homologues are both functional and produce proteins (VP6) of different sizesâsomething that has not been reported in any other orbivirus species to date. Sequence analysis suggests that eastern and western versions of segment 9 (VP6) have likely evolved as a response to adaptive selection in different geographical regions via gene duplication and subsequent mutation. These significant findings are considered unusual given the conserved nature of VP6 and its presumed role as the viral helicase. It is not currently known what the biological relevance of each homologue is to the virus.
- Subjects
AUSTRALIA; JAPAN; COMMUNICABLE diseases in animals; HEMORRHAGIC diseases; DOUBLE-stranded RNA; VIRAL proteins; RNA viruses; VIRAL variation
- Publication
Virus Genes, 2010, Vol 40, Issue 1, p67
- ISSN
0920-8569
- Publication type
Article