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- Title
T-cell colonies recognize antigen in association with specific epitopes on Ia molecules.
- Authors
Clark, Robert B.; Chiba, Joe; Zweig, Stephen E.; Shevach, Ethan M.
- Abstract
The products of the I region of the major histocompatibility complex have a role in many immunological processes and are intimately involved with, if not identical to, the genes controlling specific immune responses (Ir). Alloantibodies to I region-associated (Ia) antigens have proved extremely useful in defining the relationship between la antigens and Ir gene product function1. We have recently reported data which suggest that monoclonal anti-Ia antibodies interact with different epitopes (antigenic determinants) on macrophage Ia molecules and that distinct epitopes, though on the same Ia molecule, are recognized by T cells in association with different antigens or antigen fragments2. We have now used these monoclonal xenogeneic antibodies to guinea pig Ia antigens together with antigen-specific T-cell colonies to analyse further the role of macrophage Ia antigens in T-cell activation. We demonstrate here that the proliferative responses of individual antigen-specific T-cell colonies were often completely inhibited by only one of several anti-Ia monoclonal antibodies. These results strongly support the notion that monoclonal anti-Ia antibodies can be used to define epitopes on Ia molecules that have unique functions in the activation of antigen-specific T cells, and that individual T-cell colonies recognize antigen in association with functionally distinct epitopes on Ia molecules.
- Publication
Nature, 1982, Vol 295, Issue 5848, p412
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/295412a0