We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Induction of sialyl-Lex expression by herpes simplex virus type 1 is dependent on viral immediate early RNA-activated transcription of host fucosyltransferase genes.
- Authors
Kristina Nyström; Rickard Nordén; Isabella Muylaert; Per Elias; Göran Larson; Sigvard Olofsson
- Abstract
We have previously shown that varicella-zoster virus (VZV) and cytomegalovirus (CMV) infection of diploid human fibroblasts (HEL) results in neo-expression of Lewis antigens sialyl Lewis x (sLex) and Lewis y (Ley), respectively, after transcriptional activation of different combinations of dormant human fucosyltransferase genes (FUT1, FUT3, FUT5, and FUT6), whose gene products are responsible for the synthesis of Le antigens. Here, we show that herpes simplex virus type 1 (HSV-1) also induces sLex expression dependent on induction of FUT3, FUT5, and FUT6 transcription in infected cells. HSV-1 induction of FUT5 was subsequently used as a model system for analyzing the mechanism of viral activation of dormant fucosyltransferase genes. We show that this is a rapid process, which gives rise to elevated FUT5 RNA levels already at 90 min postinfection. Augmented FUT5 transcription was found to be dependent on transcription of viral genes, but not dependent on the immediate early proteins ICP0 and ICP4, as demonstrated by experiments with HSV-1 mutants defective in expression of these genes. Augmented FUT5 transcription takes place in cycloheximide-treated HSV-1-infected cells, suggesting a more direct role for IE viral RNA during activation of cellular FUT5.
- Subjects
GENETIC transcription regulation; HERPES simplex virus; GENETICS of virus diseases; RNA; FUCOSYLTRANSFERASES; HOST-virus relationships
- Publication
Glycobiology, 2009, Vol 19, Issue 8, p847
- ISSN
0959-6658
- Publication type
Article
- DOI
10.1093/glycob/cwp057