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- Title
Temozolomide increases MHC-I expression via NF-κB signaling in glioma stem cells.
- Authors
Zhang, Dongyong; Qiu, Bo; Wang, Yunjie; Guan, Yanlei; Zhang, Luyang; Wu, Anhua
- Abstract
Gliomas are the most common and primary tumors of the central nervous system in adults. Temozolomide (TMZ) is the main drug used to treat glioma; however, prognosis remains poor for most patients. Glioma stem cells (GSCs) are thought to enable glioma initiation and evasion from immune surveillance; their immunogenicity can be determined by expression of major histocompatibility complex (MHC)-I. The present study investigated the effect of TMZ on MHC-I expression in GSCs. Glioma spheres were cultured in serum-free medium containing epidermal growth factor, basic fibroblast growth factor, and B27; MHC-I expression was detected by immunocytochemistry, quantitative real-time PCR, and flow cytometry. Nuclear factor (NF)-κB expression in glioma stem cells was detected by Western blot. TMZ enhanced MHC-I expression in GSCs, and NF-κB was activated. TMZ treatment increased MHC-I expression via modulation of NF-κB signaling in GSCs. In addition to being a chemotherapeutic agent, TMZ may also serve as an immunomodulatory agent in the treatment of glioma patients.
- Subjects
GLIOMAS; CENTRAL nervous system tumors; NUCLEAR factor of activated T-cells; CANCER chemotherapy; WESTERN immunoblotting
- Publication
Cell Biology International, 2017, Vol 41, Issue 6, p680
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1002/cbin.10773