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- Title
The effects of Bifidobacterium on the gut microbiome of macaques with chronic diarrhea.
- Authors
SONG Jia-Rong; SHANG Ke; LEI Guang-Lun; LIU Xu; YANG Sheng-Zhi; HU Gang; YUE Bi-Song; FAN Zhen-Xin
- Abstract
This study aims to explore the effects of Bifidobacterium spp on intestinal microbial diversity and diarrhea symptoms of Macaca mulatto, in chronic diarrhea with high-throughput sequencing and plate counting experiments. The relative abundance of added bifidobacteria was significantly higher than that of the unaddressed group (P<0.05) by comparative analysis of the 16S rRNA high-throughput sequencing. The feed was fed to the chronic diarrhea macaques, and then the feces were collected for metagenomic sequencing a month later, and compared with the intestinal metagenomic data of the unaddressed group of chronic diarrhea and healthy macaques which were obtained in the previous study. It was found that the relative abundance of Lactobacillus spp in the intestinal flora of the added group was significantly upregulated compared with that of the unaddressed group (P<0.05). And there was no significant difference with the healthy group (P>0.05). Finally, the plate counting experiment of Lactobacillus was performed on the faces of improved macaques and persistent diarrhea. It was found that the number of Lactobacillus in the intestines of macaques increased after feeding, validating the result of metagenomic sequencing analysis.
- Subjects
GUT microbiome; MACAQUES; BIFIDOBACTERIUM; DIARRHEA; NUCLEOTIDE sequencing; MICROBIAL diversity
- Publication
Journal Of Sichuan University (Natural Sciences Division) / Sichuan Daxue Xuebao-Ziran Kexueban, 2022, Vol 59, Issue 6, p1
- ISSN
0490-6756
- Publication type
Article
- DOI
10.19907/j.0490-6756.2022.066003